An improved butanol-HCl assay for quantification of water-soluble, acetone:methanol-soluble, and insoluble proanthocyanidins (condensed tannins)
نویسندگان
چکیده
منابع مشابه
Rapid quantification of proanthocyanidins (condensed tannins) with a continuous flow analyzer.
Proanthocyanidins (condensed tannins) frequently need to be quantified in large numbers of samples in food, plant, and environmental studies. An automated colorimetric method to quantify proanthocyanidins with sulfuric acid (H(2)SO(4)) was therefore developed for use in a continuous flow analyzer. Assay conditions were optimized using 50% methanol extracts of paper birch, sugar maple, and quaki...
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The butanol-HCl spectrophotometric assay is widely used for quantifying extractable and insoluble condensed tannins (CT, syn. proanthocyanidins) in foods, feeds, and foliage of herbaceous and woody plants, but the method underestimates total CT content when applied directly to plant material. To improve CT quantitation, we tested various cosolvents with butanol-HCl and found that acetone increa...
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The soluble, total-bound and ®bre-bound condensed tannins (CT) in Cassia rotundifolia (cassia), Lablab purpureus (lablab) and Macroptilium atropurpureum (siratro) grown in tropical and subtropical regions were determined by the butanol±HCl method. Cassia had the highest total CT content of 29.5 g/kg DM than that of lablab, 16.9 g/kg DM which was intermediate while that of siratro, 12.4 g/kg DM,...
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ژورنال
عنوان ژورنال: Plant Methods
سال: 2017
ISSN: 1746-4811
DOI: 10.1186/s13007-017-0213-3